Plasmid

Part:BBa_K4362019:Design

Designed by: Liuziteng Wang   Group: iGEM22_Tianjin   (2022-09-08)


Micro Venus-hyg


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 774
    Illegal XbaI site found at 5980
    Illegal SpeI site found at 3041
    Illegal SpeI site found at 5315
    Illegal SpeI site found at 7785
    Illegal SpeI site found at 8292
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 774
    Illegal NheI site found at 4175
    Illegal NheI site found at 5073
    Illegal SpeI site found at 3041
    Illegal SpeI site found at 5315
    Illegal SpeI site found at 7785
    Illegal SpeI site found at 8292
    Illegal PstI site found at 864
    Illegal NotI site found at 2534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 774
    Illegal BglII site found at 3028
    Illegal BglII site found at 5983
    Illegal BglII site found at 6078
    Illegal BamHI site found at 8282
    Illegal XhoI site found at 1558
    Illegal XhoI site found at 2510
    Illegal XhoI site found at 3220
    Illegal XhoI site found at 3990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 774
    Illegal XbaI site found at 5980
    Illegal SpeI site found at 3041
    Illegal SpeI site found at 5315
    Illegal SpeI site found at 7785
    Illegal SpeI site found at 8292
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 774
    Illegal XbaI site found at 5980
    Illegal SpeI site found at 3041
    Illegal SpeI site found at 5315
    Illegal SpeI site found at 7785
    Illegal SpeI site found at 8292
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 2167
    Illegal AgeI site found at 7178
    Illegal AgeI site found at 7565
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8234
    Illegal BsaI.rc site found at 9670
    Illegal SapI site found at 8587
    Illegal SapI site found at 10691


Design Notes

This sequence is a code based on our first model in Saccharomyces cerevisiae


Source

From our first coding model

References